Anti-Hu CD300a Purified

CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

HPB human acute lymphoid leukemia cell line

Target Antigen

CD300a

Clone

MEM-260

Applications

FC (QC tested), WB

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Carnec X, Meertens L, Dejarnac O, Perera-Lecoin M, Hafirassou ML, Kitaura J, Ramdasi R, Schwartz O, Amara A: The Phosphatidylserine and Phosphatidylethanolamine Receptor CD300a Binds Dengue Virus and Enhances Infection. J Virol. 2015 Oct 14;90 (1) :92-102. , URL: https://www.ncbi.nlm.nih.gov/pubmed/26468529 , *Sabato V, Verweij MM, Bridts CH, Levi-Schaffer F, Gibbs BF, De Clerck LS, Schiavino D, Ebo DG: CD300a is expressed on human basophils and seems to inhibit IgE/Fcε RI-dependent anaphylactic degranulation. Cytometry B Clin Cytom. 2012 May;82 (3) :132-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/22173928

Other References

*Alvarez Y, Tang X, Coligan JE, Borrego F: The CD300a (IRp60) inhibitory receptor is rapidly up-regulated on human neutrophils in response to inflammatory stimuli and modulates CD32a (FcgammaRIIa) mediated signaling. Mol Immunol. 2008 Jan;45 (1) :253-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17588661?ordin, *Dimasi N, Roessle M, Moran O, Candiano G, Svergun DI, Biassoni R: Molecular analysis and solution structure from small-angle X-ray scattering of the human natural killer inhibitory receptor IRp60 (CD300a) . Int J Biol Macromol. 2007 Feb 20;40 (3) :193-200., URL: https://www.ncbi.nlm.nih.gov/pubmed/16949664?ordin, *Bachelet I, Munitz A, Levi-Schaffer F: Abrogation of allergic reactions by a bispecific antibody fragment linking IgE to CD300a. J Allergy Clin Immunol. 2006 Jun;117 (6) :1314-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/16750992?ordin, *Bachelet I, Munitz A, Moretta A, Moretta L, Levi-Schaffer F: The inhibitory receptor IRp60 (CD300a) is expressed and functional on human mast cells. J Immunol. 2005 Dec 15;175 (12) :7989-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/16339535?ordin, *Cantoni C, Bottino C, Augugliaro R, Morelli L, Marcenaro E, Castriconi R, Vitale M, Pende D, Sivori S, Millo R, Biassoni R, Moretta L, Moretta A: Molecular and functional characterization of IRp60, a member of the immunoglobulin superfamily that functions as an inhibitory receptor in human NK cells. Eur J Immunol. 1999 Oct;29 (10) :3148-59., URL: https://www.ncbi.nlm.nih.gov/pubmed/10540326?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-260 reacts with an extracellular epitope of CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.

Applications Notes

Flow cytometry: Recommended dilution: 2 μg/ml. Western blotting: Positive material: HPB cell line, negative material: JURKAT human cell line, RAJI human cell line; non-reducing conditions.