Anti-Hu CD45R0 Purified

CD45R0 is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45R0 is expressed e.g. on macrophages, CD8+ T cells, activated T cells and myeloma cells.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Human IL-2 dependent T cells

Target Antigen

CD45R0

Clone

UCHL1

Applications

FC (QC tested), IP, IHC-P, IHC-F

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Norton AJ, Ramsay AD, Smith SH, Beverley PC, Isaacson PG: Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. J Clin Pathol. 1986 Apr;39 (4) :399-405. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2422219?dopt=A, *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995) ., URL: , *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989) ., URL: , *Merkenschlager M, Terry L, Edwards R, Beverley PC: Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. Eur J Immunol. 1988 Nov;18 (11) :1653-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/2974420?dopt=A, *Beverley PC, Merkenschlager M, Terry L: Phenotypic diversity of the CD45 antigen and its relationship to function. Immunol Suppl. 1988;1:3-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/2968306?dopt=A, *Terry LA, Brown MH, Beverley PC: The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45. Immunology. 1988 Jun;64 (2) :331-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/2455685?dopt=A, *Akbar AN, Terry L, Timms A, Beverley PC, Janossy G: Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J Immunol. 1988 Apr 1;140 (7) :2171-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/2965180?dopt=A, *Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987) ., URL: , *Beverley PC.: Human T cell subsets. Immunol Lett. 1987 Apr;14 (4) :263-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/2953677?dopt=A, *Smith SH, Brown MH, Rowe D, Callard RE, Beverley PC: Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology. 1986 May;58 (1) :63-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/2423439?dopt=A, *Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20 (8) :1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/

Other References

*Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105 (8) :3295-302., URL: https://www.ncbi.nlm.nih.gov/pubmed/15626731?ordin, *Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16 (4) :256-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/17107594?ordin, *Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176 (6) :3417-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/16517710?ordin, *Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44 (15) :3682-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/17524482?ordin, *Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19 , URL: https://www.ncbi.nlm.nih.gov/pubmed/18157742?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody UCHL1 recognizes an extracellular epitope of CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA) . The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes.

Applications Notes

Immunohistochemistry (paraffin sections) : This product does not require protein digestion pretreatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Positive tissue: tonsil. Immunohistochemistry (frozen sections) : Positive tissue: tonsil. Flow cytometry: Recommended dilution: 10 μg/ml.