Anti-STIM1 Purified

STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.

Product Specifications

Certification

RUO

Reactivity

Rat, Human, Mouse

Immunogen

Synthetized peptide (C-terminal cytoplasmic part of STIM1) .

Target Antigen

STIM1

Clone

CDN3H4

Applications

IP, WB, IHC-P, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Hájková Z, Bugajev V, Dráberová E, Vinopal S, Dráberová L, Janáček J, Dráber P, Dráber P: STIM1-directed reorganization of microtubules in activated mast cells. J Immunol. 2011 Jan 15;186 (2) :913-23., URL: http://www.ncbi.nlm.nih.gov/pubmed/21160048

Other References

*Oh-Hora M, Yamashita M, Hogan PG, Sharma S, Lamperti E, Chung W, Prakriya M, Feske S, Rao A: Dual functions for the endoplasmic reticulum calcium sensors STIM1 and STIM2 in T cell activation and tolerance. Nat Immunol. 2008 Mar 9 [Epub ahead of print] , URL: https://www.ncbi.nlm.nih.gov/pubmed/18327260?ordin, *Grigoriev I, Gouveia SM, van der Vaart B, Demmers J, Smyth JT, Honnappa S, Splinter D, Steinmetz MO, Putney JW Jr, Hoogenraad CC, Akhmanova A: STIM1 is a MT-plus-end-tracking protein involved in remodeling of the ER. Curr Biol. 2008 Feb 12;18 (3) :177-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/18249114?ordin, *Hewavitharana T, Deng X, Soboloff J, Gill DL: Role of STIM and Orai proteins in the store-operated calcium signaling pathway. Cell Calcium. 2007 Aug;42 (2) :173-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/17602740?ordin, *Cai X: Molecular evolution and functional divergence of the Ca2+ sensor protein in store-operated Ca2+ entry: stromal interaction molecule. PLoS ONE. 2007 Jul 11;2 (7) :e609., URL: https://www.ncbi.nlm.nih.gov/pubmed/17622354?ordin, *Peel SE, Liu B, Hall IP: A key role for STIM1 in store operated calcium channel activation in airway smooth muscle. Respir Res. 2006 Sep 20;7:119., URL: https://www.ncbi.nlm.nih.gov/pubmed/16987424?ordin, *Soboloff J, Spassova MA, Tang XD, Hewavitharana T, Xu W, Gill DL: Orai1 and STIM reconstitute store-operated calcium channel function. J Biol Chem. 2006 Jul 28;281 (30) :20661-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/16766533?ordin, *Williams RT, Manji SS, Parker NJ, Hancock MS, Van Stekelenburg L, Eid JP, Senior PV, Kazenwadel JS, Shandala T, Saint R, Smith PJ, Dziadek MA: Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins. Biochem J. 2001 Aug 1;357 (Pt 3) :673-85. , URL: https://www.ncbi.nlm.nih.gov/pubmed/11463338?dopt=, *Feske S: ORAI1 and STIM1 deficiency in human and mice: roles of store-operated Ca2+ entry in the immune system and beyond. Immunol Rev. 2009 Sep;231 (1) :189-209., URL: http://www.ncbi.nlm.nih.gov/pubmed/19754898?itool=

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody CDN3H4 reacts with a cytoplasmic epitope of human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.

Applications Notes

Immunocytochemistry: Methanol-aceton fixation; positive control: HeLa human cervix carcinoma cell line.Immunohistochemistry (paraffin sections) : Recommended dilution: 5 μg/ml.Western blotting: Recommended dilution: 1 μg/ml; positive control: RBL rat basophilic leukemia cell line; both reducing and non-reducing conditions.