Anti-Hu CD2 Purified

CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Normal human blood lymphocytes.

Target Antigen

CD2

Clone

LT2

Applications

IP, FC (QC tested)

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Filatov AV, Bachurin PS, Markova NA, Surnakova NE: [A panel of monoclonal antibodies against human lymphocyte antigens] Eksp Onkol. 1989;11 (2) :28-32., URL: https://pubmed.ncbi.nlm.nih.gov/2737128/, *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997) ., URL: , *Gannon GA, Rhind SG, Suzui M, Zamecnik J, Sabiston BH, Shek PN, Shephard RJ: beta-Endorphin and natural killer cell cytolytic activity during prolonged exercise. Is there a connection? Am J Physiol. 1998 Dec;275 (6 Pt 2) :R1725-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/9843861?itool=, *Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106 (10) :3567-74. , URL: https://www.ncbi.nlm.nih.gov/pubmed/16046525?itool

Other References

*Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13 (31) :4207-13., URL: https://pubmed.ncbi.nlm.nih.gov/17696249/, *Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE: Mechanisms of cellular avidity regulation in CD2-CD58-mediated T cell adhesion. ACS Chem Biol. 2006 Nov 21;1 (10) :649-58., URL: https://pubmed.ncbi.nlm.nih.gov/17168569/, *Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass-supported planar bilayers reconstituted with the immunoglobulin-related adhesion molecule CD58. J Biol Chem. 1997 Jun 20;272 (25) :15782-8., URL: https://pubmed.ncbi.nlm.nih.gov/9188475/, *Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13 (31) :4207-13., URL: https://pubmed.ncbi.nlm.nih.gov/17696249/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody LT2 reacts with an extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.