Anti-ESAT-6 (M. tuberculosis) Purified
The Mycobacterium tuberculosis antigen EsaT-6 (6 kDa early secretory antigen target EsxA) causes induction of neutrophil necrosis. This protein acts as a leukocidin, which may facilitate bacterial avoidance of the antimicrobial action of the immune system.
Product Specifications
Certification
RUO
Reactivity
Mycobacterium tuberculosis
Immunogen
EsaT-6 (Rv3875) of Mycobacterium tuberculosis
Target Antigen
ESAT-6 (M. tuberculosis)
Clone
PAb (491)
Applications
WB
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Other References
*Francis RJ, Butler RE, Stewart GR: Mycobacterium tuberculosis ESAT-6 is a leukocidin causing Ca2+ influx, necrosis and neutrophil extracellular trap formation. Cell Death Dis. 2014 Oct 16;5:e1474. , URL: http://www.ncbi.nlm.nih.gov/pubmed/25321481, , *Arlehamn CS, Sidney J, Henderson R, Greenbaum JA, James EA, Moutaftsi M, Coler R, McKinney DM, Park D, Taplitz R, Kwok WW, Grey H, Peters B, Sette A: Dissecting mechanisms of immunodominance to the common tuberculosis antigens ESAT-6, CFP10, Rv2031c (hspX), Rv2654c (TB7.7), and Rv1038c (EsxJ) . J Immunol. 2012 May 15;188 (10) :5020-31., URL: https://pubmed.ncbi.nlm.nih.gov/22504645/
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The rabbit polyclonal antibody to EsaT-6 recognizes the EsaT-6 (Rv3875) protein of Mycobacterium tuberculosis and Mycobacterium bovis.
Applications Notes
Western blotting: Recommended dilution 0.5-1 μg/ml, reducing conditions.
Isotype
Rabbit polyclonal
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