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Anti-CFP-10 (M. tuberculosis) Purified

The Mycobacterium tuberculosis antigen CFP-10 (10 kDa culture filtrate antigen EsxB; LHP), also known as Rv3874, plays important role in mycobacterial virulence. It forms a complex with EsaT-6 protein.

Product Specifications

Certification

RUO

Reactivity

Mycobacterium tuberculosis

Immunogen

CFP10 (Rv3874) of Mycobacterium tuberculosis

Target Antigen

CFP-10 (M. tuberculosis)

Clone

PAb (490)

Applications

WB

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Other References

*Yang H, Chen H, Liu Z, Ma H, Qin L, Jin R, Zheng R, Feng Y, Cui Z, Wang J, Liu J, Hu Z: A novel B-cell epitope identified within Mycobacterium tuberculosis CFP10/ESAT-6 protein. PLoS One. 2013;8 (1) :e52848., URL: https://pubmed.ncbi.nlm.nih.gov/23308124/, *Arlehamn CS, Sidney J, Henderson R, Greenbaum JA, James EA, Moutaftsi M, Coler R, McKinney DM, Park D, Taplitz R, Kwok WW, Grey H, Peters B, Sette A: Dissecting mechanisms of immunodominance to the common tuberculosis antigens ESAT-6, CFP10, Rv2031c (hspX), Rv2654c (TB7.7), and Rv1038c (EsxJ) . J Immunol. 2012 May 15;188 (10) :5020-31., URL: https://pubmed.ncbi.nlm.nih.gov/22504645/, *Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Krogh A, McLean J, Moule S, Murphy L, Oliver K, Osborne J, Quail MA, Rajandream MA, Rogers J, Rutter S, Seeger K, Skelton J, Squares R, Squares S, Sulston JE, Taylor K, Whitehead S, Barrell BG: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 1998 Jun 11;393 (6685) :537-44. Erratum in: Nature 1998 Nov 12;396 (6707) :190., URL: https://www.ncbi.nlm.nih.gov/pubmed/9634230?dopt=A

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The rabbit polyclonal antibody to CFP10 recognizes the CFP10 (Rv3874) protein of Mycobacterium tuberculosis and Mycobacterium bovis.

Applications Notes

Western blotting: Recommended dilution 0.5-1 μg/ml, reducing conditions.

Isotype

Rabbit polyclonal

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