Anti-Orf1 (N. meningitidis) Purified
Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.
Product Specifications
Certification
RUO
Reactivity
Neisseria meningitidis
Immunogen
Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/mL of aluminum hydroxide in PBS, and used to immunize a rabbit.
Target Antigen
Orf1 (N. meningitidis)
Clone
PAb (489)
Applications
WB
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Prochazkova K, Osicka R, Linhartova I, Halada P, Sulc M, Sebo P: The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC. J Biol Chem. 2005 Feb 4;280 (5) :3251-8. Epub 2004 Nov 2. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15525636?dopt=
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.
Applications Notes
Western blotting: Recommended dilution 0.5-1 μg/ml, reducing conditions.
Isotype
Rabbit polyclonal
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