Anti-CRP Purified

The C-reactive protein (CRP) is a cyclic pentameric pentraxin family acute phase protein composed of five identical noncovalently bound nonglycosylated subunits (each subunit 24 kDa; physiologic CRP molecule 117,5 kDa) . CRP is produced by the liver and its plasma levels rise dramatically during inflammatory processes occuring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.) and is also believed to play an important role in innate immunity. Patients with elevated basal levels of CRP are at increased risk for hypertension and cardiovascular disease.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Purified human CRP

Target Antigen

CRP

Clone

Applications

ELISA

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Krämer PM, Kess M, Kremmer E, Schulte-Hostede S: Multi-parameter determination of TNFα , PCT and CRP for point-of-care testing. Analyst. 2011 Feb 21;136 (4) :692-5., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=kremmer+e+schulte-hostede+s

Other References

*van der Harst P, Voors AA, Volbeda M, Buikema H, van Veldhuisen DJ, van Gilst WH: Usefulness of preoperative C-reactive protein and soluble intercellular adhesion molecule-1 level for predicting future cardiovascular events after coronary artery bypass grafting. Am J Cardiol. 2006 Jun 15;97 (12) :1697-701., URL: https://pubmed.ncbi.nlm.nih.gov/16765116/, *Meyer MH, Hartmann M, Krause HJ, Blankenstein G, Mueller-Chorus B, Oster J, Miethe P, Keusgen M: CRP determination based on a novel magnetic biosensor. Biosens Bioelectron. 2007 Jan 15;22 (6) :973-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=hartmann+m+blankenstein

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody C5 recognizes human CRP, a 117 kDa plasma acute phase protein; it recognizes antigen both in the presence and in the absence (samples containing EDTA) of Ca2+.

Applications Notes

ELISA: The antibody C5 has been tested as the capture antibody in a sandwich ELISA for analysis of human C-Reactive Protein in combination with the antibody C7 (cat. no. 1B-484-C100) .