Anti-Hu CD66e Purified

The CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3) . Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Human carcinoembryonic antigen (CEA; CEACAM5)

Target Antigen

CD66e

Clone

CB30

Applications

FC (QC tested), IP, IHC-P, IHC-F

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Koga S, Oshima Y, Honkura N, Iimura T, Kameda K, Sato K, Yoshida M, Yamamoto Y, Watanabe Y, Hikita A, Imamura T: In vivo subcellular imaging of tumors in mouse models using a fluorophore-conjugated anti-carcinoembryonic antigen antibody in two-photon excitation microscopy. Cancer Sci. 2014 Oct;105 (10) :1299-306., URL: https://pubmed.ncbi.nlm.nih.gov/25117702/, *Wang L, Ma N, Okamoto S, Amaishi Y, Sato E, Seo N, Mineno J, Takesako K, Kato T, Shiku H: Efficient tumor regression by adoptively transferred CEA-specific CAR-T cells associated with symptoms of mild cytokine release syndrome. Oncoimmunology. 2016 Jul 25;5 (9) :e1211218., URL: https://pubmed.ncbi.nlm.nih.gov/27757303/

Other References

*Oikawa S, Nakazato H, Kosaki G: Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence. Biochem Biophys Res Commun. 1987 Jan 30;142 (2) :511-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/3814146?dopt=A, *Thompson JA, Grunert F, Zimmermann W: Carcinoembryonic antigen gene family: molecular biology and clinical perspectives. J Clin Lab Anal. 1991;5 (5) :344-66. Review., URL: https://www.ncbi.nlm.nih.gov/pubmed/1941355?dopt=a, *Gangopadhyay A, Bajenova O, Kelly TM, Thomas P: Carcinoembryonic antigen induces cytokine expression in Kuppfer cells: implications for hepatic metastasis from colorectal cancer. Cancer Res. 1996 Oct 15;56 (20) :4805-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/8841002?dopt=A, *Chen CJ, Li LJ, Maruya A, Shively JE: In vitro and in vivo footprint analysis of the promoter of carcinoembryonic antigen in colon carcinoma cells: effects of interferon gamma treatment. Cancer Res. 1995 Sep 1;55 (17) :3873-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/7641207?dopt=A

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody CB30 recognizes CD66e (CEA; 180-200 kDa), an extracellular cell surface-bound carcinoembryonic antigen mainly expressed on epithelial cells.

Applications Notes

Immunohistochemistry (paraffin sections) : Staining technique: standard ABC technique (DAB+), recommended dilution: 10 μg/ml (1:100), positive tissue: adenocarcinoma of colon, pretreatment: 0,1% pepsin in 0,1 M HCl for 30 min at room temperature.