Anti-GST tag Purified
The GST (glutathione-S-transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors.
Product Specifications
Certification
RUO
Reactivity
Tagged fusion proteins in all species
Immunogen
GST fusion protein
Target Antigen
GST tag
Clone
S-tag-05
Applications
WB, IP
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibod is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.
Applications Notes
Western blotting: Recommended dilution: 1 μg/ml; positive control: GST fusion protein, reducing conditions.
Isotype
Mouse IgG2b
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