Anti-GFP Purified
Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (λ =509 nm) when excited (excitation by Blue or UV light, absorption peak λ =395 nm) . GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. yellow fluorescent protein, cyan fluorescent protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.
Product Specifications
Certification
RUO
Reactivity
Tagged fusion proteins in all species
Immunogen
EGFP, a native full-length protein
Target Antigen
GFP
Clone
PAb (476)
Applications
IP, WB, ICC
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.
Applications Notes
Isotype
Rabbit polyclonal
Frequently Asked Questions
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