Anti-MAP2 Purified
MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.
Product Specifications
Certification
RUO
Reactivity
Pig, Human, Mouse
Immunogen
Microtubule protein (bovine brain) enriched for kinesin
Target Antigen
MAP2
Clone
MT-08
Applications
IP, WB, IHC-P, IHC-F, ICC, ELISA
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Ordureau A, Paulo JA, Zhang J, An H, Swatek KN, Cannon JR, Wan Q, Komander D, Harper JW: Global landscape and dynamics of Parkin and USP30-dependent ubiquitylomes in iNeurons during mitophagic signaling. Mol Cell. 2020 Mar 5;77 (5) :1124-1142., URL: https://pubmed.ncbi.nlm.nih.gov/32142685/
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The antibody MT-08 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab), an intracellular antigen.
Applications Notes
Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: brain. Immunohistochemistry (frozen sections) : Positive tissue: murine brain. Immunoprecipitation: Positive material: porcine brain. Western blotting: Positive control: porcine brain. ELISA: Positive control: porcine brain. Immunocytochemistry: Positive control: human neuroblastoma SH-SY5Y.
Isotype
Mouse IgG1
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