Anti-Hu p53 (pS392) Purified

The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53.

Target Antigen

P53 (pS392)

Clone

FP3.2 [FPS392]

Applications

WB, IHC-P

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Blaydes JP, Craig AL, Wallace M, Ball HM, Traynor NJ, Gibbs NK, Hupp TR: Synergistic activation of p53-dependent transcription by two cooperating damage recognition pathways. Oncogene. 2000 Aug 10;19 (34) :3829-39., URL: https://www.ncbi.nlm.nih.gov/pubmed/10951576?dopt=, *Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15 (11) :909-912., URL: https://www.ncbi.nlm.nih.gov/pubmed/30377371 , *Koutová D, Havelek R, Peterová E, Muthná D, Královec K, Breiterová K, Cahlíková L, Řezáčová M: Pancracine, a montanine-type amaryllidaceae alkaloid, inhibits proliferation of A549 lung adenocarcinoma cells and induces apoptotic cell death in MOLT-4 leukemic cells. Int J Mol Sci. 2021 Jun 29;22 (13) :7014., URL: https://pubmed.ncbi.nlm.nih.gov/34209868/

Other References

*Tanigawa S, Fujii M, Hou DX: Stabilization of p53 is involved in quercetin-induced cell cycle arrest and apoptosis in HepG2 cells. Biosci Biotechnol Biochem. 2008 Mar;72 (3) :797-804., URL: https://www.ncbi.nlm.nih.gov/pubmed/18323654?ordin, *Taylor WR, Agarwal ML, Agarwal A, Stacey DW, Stark GR: p53 inhibits entry into mitosis when DNA synthesis is blocked. Oncogene. 1999 Jan 14;18 (2) :283-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/9927185?ordina, *Taylor WR, DePrimo SE, Agarwal A, Agarwal ML, Schönthal AH, Katula KS, Stark GR: Mechanisms of G2 arrest in response to overexpression of p53. Mol Biol Cell. 1999 Nov;10 (11) :3607-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/10564259?ordin, *Agarwal ML, Agarwal A, Taylor WR, Chernova O, Sharma Y, Stark GR: A p53-dependent S-phase checkpoint helps to protect cells from DNA damage in response to starvation for pyrimidine nucleotides. Proc Natl Acad Sci U S A. 1998 Dec 8;95 (25) :14775-80., URL: https://www.ncbi.nlm.nih.gov/pubmed/9843965?ordina, *Agarwal ML, Agarwal A, Taylor WR, Stark GR: p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts. Proc Natl Acad Sci U S A. 1995 Aug 29;92 (18) :8493-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/7667317?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody FP3.2 [FPS392] reacts with human p53 tumour suppressor intracellular protein phosphorylated at CKII site (Ser 392) .

Applications Notes

Immunohistochemistry (paraffin sections) : Standard ABC technique (DAB+), pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0, recommended dilution: 10 μg/ml, incubation: 1 hour at RT; or overnight at 4°C, positive tissue: breast carcinoma with high level of wild-type p53. Western blotting: recommended dilution: 1 μg/ml.