Anti-Hu beta2-Microglobulin Purified

*Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98 (5) :1561-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/11520808?dopt=, *Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha) . 1984;30 (6) :369-76., URL: https://www.ncbi.nlm.nih.gov/pubmed/6394369?dopt=A

The mouse monoclonal antibody B2M-02 reacts with beta2-microglobulin (beta2M), an extracellular antigen associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

Immunohistochemistry (paraffin sections) : Heat-mediated antigen retrieval; recommended dilution: 5 μg/ml; positive tissue: liver. Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions preferred. Flow cytometry: Recommended dilution: 1 μg/ml; positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes. ELISA: Working dilution should be determinated by investigator.