Anti-Hu CD62L Purified

CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1) .

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Peripheral blood leukocytes

Target Antigen

CD62L

Clone

LT-TD180

Applications

FC (QC tested)

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8;113 (2) :358-69., URL: https://www.ncbi.nlm.nih.gov/pubmed/18922855, *Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260 (FTO) . J Biomed Biotechnol. 2010;2010:841451., URL: https://www.ncbi.nlm.nih.gov/pubmed/20339516, *Simone R, Zicca A, Saverino D: The frequency of regulatory CD3+CD8+CD28- CD25+ T lymphocytes in human peripheral blood increases with age. J Leukoc Biol. 2008 Dec;84 (6) :1454-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/18780874

Other References

*Ramachandran V, Williams M, Yago T, Schmidtke DW, McEver RP: Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin. Proc Natl Acad Sci U S A. 2004 Sep 14;101 (37) :13519-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/15353601?ordin, *Ding Z, Issekutz TB, Downey GP, Waddell TK: L-selectin stimulation enhances functional expression of surface CXCR4 in lymphocytes: implications for cellular activation during adhesion and migration. Blood. 2003 Jun 1;101 (11) :4245-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/12609846?ordin, *Simon SI, Burns AR, Taylor AD, Gopalan PK, Lynam EB, Sklar LA, Smith CW: L-selectin (CD62L) cross-linking signals neutrophil adhesive functions via the Mac-1 (CD11b/CD18) beta 2-integrin. J Immunol. 1995 Aug 1;155 (3) :1502-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/7543524?ordina, *von Andrian UH, Chambers JD, Berg EL, Michie SA, Brown DA, Karolak D, Ramezani L, Berger EM, Arfors KE, Butcher EC. L-selectin mediates neutrophil rolling in inflamed venules through sialyl LewisX-dependent and -independent recognition pathways. Blood. 1993 Jul 1;82 (1) :182-91., URL: https://www.ncbi.nlm.nih.gov/pubmed/7686786?ordina, *Von Andrian UH, Hansell P, Chambers JD, Berger EM, Torres Filho I, Butcher EC, Arfors KE: L-selectin function is required for beta 2-integrin-mediated neutrophil adhesion at physiological shear rates in vivo. Am J Physiol. 1992 Oct;263 (4 Pt 2) :H1034-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/1384360?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody LT-TD180 reacts with an extracellular epitope of CD62L (L-selectin), a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.