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Anti-DDDDK tag Purified

Product Specifications

Certification

RUO

Reactivity

Tagged fusion proteins in all species

Immunogen

Synthetic peptide: DYKDDDDK conjugated to KLH

Target Antigen

DDDDK tag

Clone

F-tag-01

Applications

ICC, WB

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25 (11) :2326-37., URL: https://www.ncbi.nlm.nih.gov/pubmed/16724116?dopt=, *Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription driven by the Wnt effector TCF-4. Nucleic Acids Res. 2009 Mar 20. [Epub ahead of print], URL: https://www.ncbi.nlm.nih.gov/pubmed/19304756?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody F-tag-01 recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.

Isotype

Mouse IgG1

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