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Anti-HLA Class I Purified

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Product Specifications

Certification

RUO

Negative Species

Rabbit

Reactivity

Cow, Non-human primates, Human, Cat

Immunogen

Membrane of human tonsil cells

Target Antigen

HLA Class I

Clone

W6/32

Applications

FC (QC tested), IP, IHC-F, ICC, ELISA, MC-CyTOF

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kievits F, Ivanyi P: Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin.Hum Immunol. 1987 Oct;20 (2) :115-26.</li>, URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=3316135&query_hl=24&itool=pubmed_docsum , *Ladasky JJ, Shum BP, Canavez F, Seuanez HN, Parham P: Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody. Immunogenetics. 1999 Apr;49 (4) :312-20. </li>, URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=10079295&query_hl=8&itool=pubmed_docsum , *Le Discorde M, Moreau P, Sabatier P, Legeais JM, Carosella ED: Expression of HLA-G in human cornea, an immune-privileged tissue. Hum Immunol. 2003 Nov;64 (11) :1039-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/14602233?ordin, *Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics. 2001 Aug;53 (6) :440-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/11685454?dopt=, *Shields MJ, Ribaudo RK: Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin. Tissue Antigens 1998 May;51 (5) :567-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/9672158?dopt=A, *Jacobsen, C. N. et al. (1993) : Reactivities of 20 anti-human monclonal antibodies with leucocytes from ten different animal species. Vet. Immunopathol. 39: 461 - 466., URL: https://www.ncbi.nlm.nih.gov/pubmed/8116221?dopt=A, *Kievits F, Ivanyi P: Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin.Hum Immunol. 1987 Oct;20 (2) :115-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/3316135?dopt=A, *Stern, P. et al. (1987) : Class I-like MHC molecules expressed by baboon placental synctiotrophoblast. Journal of Immunology. 138 (4) : 1088 - 1091., URL: https://www.ncbi.nlm.nih.gov/pubmed/3543121?dopt=A, *Neefjes, J.J. et al. (1986) : A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics 23: 341-347., URL: https://www.ncbi.nlm.nih.gov/pubmed/3458670?dopt=A, *Brodsky, F.M. et al. (1982) : Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics 15: 151-166., URL: https://www.ncbi.nlm.nih.gov/pubmed/6174442?dopt=A, *Barnstable, C. J., et al. (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - new tools for genetic analysis. Cell. 14: 9 - 20., URL: https://www.ncbi.nlm.nih.gov/pubmed/667938?dopt=Ab, *Polakova K, Karpatova M, Russ G: Dissociation of beta 2-microglobulin is responsible for selective reduction of HLA class I antigenicity following acid treatment of cells. Mol Immunol. 1993 Oct;30 (14) :1223-30., URL: https://pubmed.ncbi.nlm.nih.gov/7692238/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.

Applications Notes

Flow cytometry: Recommended dilution: 1-12 µg/ml

Isotype

Mouse IgG2a

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