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Anti-Blood Group Lewis b Purified

Selected Variant

11-386-C100

Size: 0.1 mg

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification) .

Target Antigen

Blood Group Lewis b

Clone

2-25LE

Applications

IHC-P, WB, ELISA

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems., URL: , *Creuzot-Garcher C, Guerzider V, Assem M, Bron AM, Delannoy P, Bara J: Alteration of sialyl Lewis epitope expression in pterygium. Invest Ophthalmol Vis Sci. 1999 Jul;40 (8) :1631-6. , URL: https://www.ncbi.nlm.nih.gov/pubmed/10393028?dopt=, *Bara J, Gautier R, Le Pendu J, Oriol R: Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J. 1988 Aug 15;254 (1) :185-93., URL: https://pubmed.ncbi.nlm.nih.gov/2460087/, *Gousset K, Schiff E, Langevin C, Marijanovic Z, Caputo A, Browman DT, Chenouard N, de Chaumont F, Martino A, Enninga J, Olivo-Marin JC, Männel D, Zurzolo C: Prions hijack tunnelling nanotubes for intercellular spread. Nat Cell Biol. 2009 Mar;11 (3) :328-36. , URL: https://pubmed.ncbi.nlm.nih.gov/19198598/, *Matza D, Kerem A, Medvedovsky H, Lantner F, Shachar I: Invariant chain-induced B cell differentiation requires intramembrane proteolytic release of the cytosolic domain. Immunity. 2002 Nov;17 (5) :549-60. , URL: https://pubmed.ncbi.nlm.nih.gov/12433362/

Other References

*Bara J, Gautier R, Le Pendu J, Oriol R: Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J. 1988 Aug 15;254 (1) :185-93. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2460087?dopt=A, *Torrado J, Correa P, Ruiz B, Bernardi P, Závala D, Bara J: Lewis antigen alterations in gastric cancer precursors. Gastroenterology. 1992 Feb;102 (2) :424-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/1732113?dopt=A

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

B

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 5-10 μg/ml; positive control: colorectal adenocarcinoma; heat mediated antigen retrieval (sodium citrate) ; mAb incubation: 1 hour / RT; detection DAB.

Isotype

Mouse IgG1

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