Anti-Blood Group Lewis a Purified
Product Specifications
Certification
RUO
Reactivity
Human
Immunogen
Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification) .
Target Antigen
Blood Group Lewis a
Clone
7LE
Applications
IHC-P, WB, ELISA
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems., URL: , *Creuzot-Garcher C, Guerzider V, Assem M, Bron AM, Delannoy P, Bara J: Alteration of sialyl Lewis epitope expression in pterygium. Invest Ophthalmol Vis Sci. 1999 Jul;40 (8) :1631-6. , URL: https://www.ncbi.nlm.nih.gov/pubmed/10393028?dopt=
Other References
*Torrado J, Correa P, Ruiz B, Bernardi P, Zavala D, Bara J: Lewis antigen alterations in gastric cancer precursors. Gastroenterology. 1992 Feb;102 (2) :424-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/1732113?dopt=A, *Bara J, Gautier R, Le Pendu J, Oriol R: Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J. 1988 Aug 15;254 (1) :185-93. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2460087?dopt=A
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
Aa
Applications Notes
Immunohistochemistry (paraffin sections) : Recommended dilution: 5 μg/ml; positive control: colorectal adenocarcinoma; heat mediated antigen retrieval (sodium citrate) ; mAb incubation 1 hour / RT, detection DAB.
Isotype
Mouse IgG1
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