Anti-alpha-Fetoprotein Purified
Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.
Product Specifications
Certification
RUO
Reactivity
Human
Immunogen
Purified alpha-Fetoprotein
Target Antigen
Alpha-Fetoprotein
Clone
AFP-11
Applications
IHC-P, ELISA, RIA, WB, ICC
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Stefanova I, Horejsi V, Kristofova H, Angelisova P, Zizkovsky V, Hilgert I: Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J Immunol Methods. 1988 Jun 28;111 (1) :67-73., URL: https://pubmed.ncbi.nlm.nih.gov/2455753/
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The mouse monoclonal antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
Applications Notes
ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100) . Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: hepatocellular carcinoma; heat mediated antigen retrieval (sodium citrate buffer) ; mAb incubation 1 hour / RT, detection DAB.Western blotting: Recommended dilution: 2 μg/ml, non-reducing conditions.
Isotype
Mouse IgG1
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