What is the catalog number for Anti-Hu CD3 Purified?

The catalog number for Anti-Hu CD3 Purified is 11-381-C100.

What sizes are available for Anti-Hu CD3 Purified?

Anti-Hu CD3 Purified is available in the following size(s): 0.1 mg.

Is Anti-Hu CD3 Purified in stock?

Anti-Hu CD3 Purified is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Anti-Hu CD3 Purified?

Anti-Hu CD3 Purified is manufactured by AFG-XBO.

What is the estimated delivery time for Anti-Hu CD3 Purified?

Estimated delivery time for Anti-Hu CD3 Purified is approximately 10 business day(s), depending on your location.

Do you offer bulk or custom quantities of this product?

Yes. Ordering two or more units, or combining this with other products, usually qualifies for a discount depending on quantity. Contact our sales team with your required volume for a custom quote.

Do you provide a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for most products upon request. Contact our support team with the lot number to obtain the relevant CoA.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

Anti-Hu CD3 Purified

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Human peripheral blood lymphocytes.

Target Antigen

CD3

Clone

MEM-92

Applications

FC (QC tested), IP

Concentration

1 mg/mL

Format

Purified

Buffer

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

References & Citations

*Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1;272 (31) :19242-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9235917, *Brdicka T, Imrich M, Angelisová P, Brdicková N, Horváth O, Spicka J, Hilgert I, Lusková P, Dráber P, Novák P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsí V: Non-T cell activation linker (NTAL) : a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196 (12) :1617-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/12486104?itool, *Stefanová I, Saville MW, Peters C, Cleghorn FR, Schwartz D, Venzon DJ, Weinhold KJ, Jack N, Bartholomew C, Blattner WA, Yarchoan R, Bolen JB, Horak ID: HIV infection--induced posttranslational modification of T cell signaling molecules associated with disease progression. J Clin Invest. 1996 Sep 15;98 (6) :1290-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/8823293?itool=, *Batista A, Millán J, Mittelbrunn M, Sánchez-Madrid F, Alonso MA: Recruitment of transferrin receptor to immunological synapse in response to TCR engagement. J Immunol. 2004 Jun 1;172 (11) :6709-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/15153487?itool, *Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198 (10) :1453-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/14610046?itool, *Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15;178 (4) :2262-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/17277131?ordin, *Souček J, Hilgert I, Budová I, Lindnerová G: Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2. Neoplasma. 1994;41 (2) :75-81., URL: https://pubmed.ncbi.nlm.nih.gov/8208318/, *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989) ., URL:

Other References

*Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279 (28) :28827-30., URL: https://pubmed.ncbi.nlm.nih.gov/15084594/, *Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7 (5) :490-5., URL: https://pubmed.ncbi.nlm.nih.gov/16670682/, *Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24 (2) :133-9., URL: https://pubmed.ncbi.nlm.nih.gov/16473826/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-92 reacts with an extracellular epitope on epsilon chain of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.