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Anti-CSK Purified

C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them it lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases, that are localized in lipid rafts, is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.

Product Specifications

Certification

RUO

Immunogen

Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450.

Target Antigen

CSK

Clone

CSK-04

Applications

WB, IP, ICC

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Palomero T, Couronné L, Khiabanian H, Kim MY, Ambesi-Impiombato A, Perez-Garcia A, Carpenter Z, Abate F, Allegretta M, Haydu JE, Jiang X, Lossos IS, Nicolas C, Balbin M, Bastard C, Bhagat G, Piris MA, Campo E, Bernard OA, Rabadan R, Ferrando AA: Recurrent mutations in epigenetic regulators, RHOA and FYN kinase in peripheral T cell lymphomas. Nat Genet. 2014 Feb;46 (2) :166-70. , URL: https://www.ncbi.nlm.nih.gov/pubmed/24413734

Other References

*Liang F, Luo Y, Dong Y, Walls CD, Liang J, Jiang HY, Sanford JR, Wek RC, Zhang ZY: Translational Control of C-terminal Src Kinase (Csk) Expression by PRL3 Phosphatase. J Biol Chem. 2008 Apr 18;283 (16) :10339-46., URL: https://www.ncbi.nlm.nih.gov/pubmed/18268019?ordin, *Ingley E: Src family kinases: regulation of their activities, levels and identification of new pathways. Biochim Biophys Acta. 2008 Jan;1784 (1) :56-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/17905674?ordin, *Takayama Y, Tanaka S, Nagai K, Okada M: Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of paxillin and FAK. J Biol Chem. 1999 Jan 22;274 (4) :2291-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9890993?ordina, *Bergman M, Joukov V, Virtanen I, Alitalo K: Overexpressed Csk tyrosine kinase is localized in focal adhesions, causes reorganization of alpha v beta 5 integrin, and interferes with HeLa cell spreading. Mol Cell Biol. 1995 Feb;15 (2) :711-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/7529872?ordina, *Chow LM, Fournel M, Davidson D, Veillette A: Negative regulation of T-cell receptor signalling by tyrosine protein kinase p50csk. Nature. 1993 Sep 9;365 (6442) :156-60., URL: https://www.ncbi.nlm.nih.gov/pubmed/8371758?ordina, *Sabe H, Okada M, Nakagawa H, Hanafusa H: Activation of c-Src in cells bearing v-Crk and its suppression by Csk. Mol Cell Biol. 1992 Oct;12 (10) :4706-13., URL: https://www.ncbi.nlm.nih.gov/pubmed/1383688?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa intracellular non-receptor protein tyrosine kinase.

Species

Human, Mouse

CAS Number

9007-83-4

Isotype

Mouse IgG1

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