Anti-LYN Purified

Lyn is a Src-family protein tyrosine kinase that is predominantly expressed in hematopoietic cells. It is associated with a number of cell surface receptors including the B cell antigen receptor (BCR) and Fc receptors. Upon their triggering, Lyn phosphorylates subunits of these receptors in a cholesterol-dependent manner, utilizing the plasma membrane lipid raft system. The phosphorylated intracellular domains of the receptors are accessible for cytoplasmic Syk tyrosine kinase, which is activated by Lyn-mediated phosphorylation and which transduces the signal to downstream adaptors. Lyn is abnormally distributed in acute myeloid leukemia cells and seems to be a novel pharmacologic target of this disease.

Product Specifications

Certification

RUO

Reactivity

Rat, Human, Mouse

Immunogen

Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238) .

Target Antigen

LYN

Clone

LYN-01

Applications

IP, WB, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Draberova L, Amoui M, Draber P.: Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane microdomains. Immunology. 1996 Jan;87 (1) :141-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/8666426?dopt=A, *Amoui M, Draberova L, Tolar P, Drber P: Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors. Eur J Immunol. 1997 Jan;27 (1) :321-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/9022035?dopt=A, *Kovarova M, Tolar P, Arudchandran R, Draberova L, Rivera J, Draber P: Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation. Mol Cell Biol. 2001 Dec;21 (24) :8318-28. , URL: https://www.ncbi.nlm.nih.gov/pubmed/11713268?dopt=

Other References

*Strzelecka-Kiliszek A, Kwiatkowska K, Sobota A: Lyn and Syk kinases are sequentially engaged in phagocytosis mediated by Fc gamma R. J Immunol. 2002 Dec 15;169 (12) :6787-94., URL: https://www.ncbi.nlm.nih.gov/pubmed/12471110?ordin, *Rivera J, Olivera A: A current understanding of Fc epsilon RI-dependent mast cell activation. Curr Allergy Asthma Rep. 2008 Mar;8 (1) :14-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/18377769?ordin, *Davey AM, Krise KM, Sheets ED, Heikal AA: Molecular perspective of antigen-mediated mast cell signaling. J Biol Chem. 2008 Mar 14;283 (11) :7117-27., URL: https://www.ncbi.nlm.nih.gov/pubmed/18093971?ordin, *Kinoshita T, Miyano N, Nakai R, Yokota K, Ishiguro H, Tada T: Protein purification and preliminary crystallographic analysis of human Lyn tyrosine kinase. Protein Expr Purif. 2008 Apr;58 (2) :318-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/18272395?ordin, *Dos Santos C, Demur C, Bardet V, Prade-Houdellier N, Payrastre B, Récher C: A critical role for Lyn in acute myeloid leukemia. Blood. 2008 Feb 15;111 (4) :2269-79., URL: https://www.ncbi.nlm.nih.gov/pubmed/18056483?ordin