Anti-FYN Purified

Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.

Product Specifications

Certification

RUO

Reactivity

Human, Mouse

Immunogen

Bacterially expressed recombinant fragment of human Fyn (aa 7-176) .

Target Antigen

FYN

Clone

FYN-01

Applications

IP, WB, IHC-P, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15;178 (4) :2262-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/17277131?ordin

Other References

*Rivera J, Olivera A: A current understanding of Fc epsilon RI-dependent mast cell activation. Curr Allergy Asthma Rep. 2008 Mar;8 (1) :14-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/18377769?ordin, *Makinodan E, Manabe T, Makinodan M, Yamauchi T, Matsuyoshi H, Sakumura R, Tatsumi K, Wanaka A. A novel role for Fyn: change in sphere formation ability in murine embryonic stem cells. Neuroscience. 2007 Jun 15;147 (1) :1-4., URL: https://www.ncbi.nlm.nih.gov/pubmed/17521824?ordin, *Samayawardhena LA, Kapur R, Craig AW: Involvement of Fyn kinase in Kit and integrin-mediated Rac activation, cytoskeletal reorganization, and chemotaxis of mast cells. Blood. 2007 May 1;109 (9) :3679-86., URL: https://www.ncbi.nlm.nih.gov/pubmed/17213284?ordin, *Filby A, Seddon B, Kleczkowska J, Salmond R, Tomlinson P, Smida M, Lindquist JA, Schraven B, Zamoyska R. Fyn regulates the duration of TCR engagement needed for commitment to effector function. J Immunol. 2007 Oct 1;179 (7) :4635-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/17878361?ordin, *Wu HY, Hsu FC, Gleichman AJ, Baconguis I, Coulter DA, Lynch DR: Fyn-mediated phosphorylation of NR2B Tyr-1336 controls calpain-mediated NR2B cleavage in neurons and heterologous systems. J Biol Chem. 2007 Jul 13;282 (28) :20075-87., URL: https://www.ncbi.nlm.nih.gov/pubmed/17526495?ordin, *Hattori K, Uchino S, Isosaka T, Maekawa M, Iyo M, Sato T, Kohsaka S, Yagi T, Yuasa S: Fyn is required for haloperidol-induced catalepsy in mice. J Biol Chem. 2006 Mar 17;281 (11) :7129-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/16407246?ordin, *Talmor-Cohen A, Tomashov-Matar R, Tsai WB, Kinsey WH, Shalgi R: Fyn kinase-tubulin interaction during meiosis of rat eggs. Reproduction. 2004 Oct;128 (4) :387-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/15454633?ordin, *Utting O, Priatel JJ, Teh SJ, Teh HS: p59fyn (Fyn) promotes the survival of anergic CD4-CD8- alpha beta TCR+ cells but negatively regulates their proliferative response to antigen stimulation. J Immunol. 2001 Feb 1;166 (3) :1540-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/11160194?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody FYN-01 recognizes Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase (intracellular antigen) .

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 5 μg/ml; positive tissue: brain. Western blotting: Recommended dilution: 2 μg/ml; positive control: JURKAT human T cell leukemia cell line. Immunoprecipitation: positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF) . The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.