Anti-NTAL Purified

NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.

Product Specifications

Certification

RUO

Negative Species

Mouse

Reactivity

Human

Immunogen

Peptide corresponding to aa 196-212 of human NTAL coupled to KLH.

Target Antigen

NTAL

Clone

NAP-03

Applications

IP, WB, IHC-P

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsi V: Non-T cell activation linker (NTAL) : a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196 (12) :1617-26. , URL: https://www.ncbi.nlm.nih.gov/pubmed/12486104?dopt=

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody NAP-03 recognizes a defined intracellular epitope (aa 196-212) of human Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein associating with lipid raft components in the plasma membrane of B cells, NK cells and myeloid cells.

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 5 μg/ml; positive tissue: tonsil, germinal center. Western blotting: Recommended dilution: 1-2 μg/ml, incubation 60 min; positive control: RAMOS human lymphoma cell line; non-reducing conditions, 12% separating SDS-PAGE gel.