Anti-NTAL Purified
NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Product Specifications
Certification
RUO
Negative Species
Mouse
Reactivity
Human
Immunogen
Peptide corresponding to aa 196-212 of human NTAL coupled to KLH.
Target Antigen
NTAL
Clone
NAP-03
Applications
IP, WB, IHC-P
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsi V: Non-T cell activation linker (NTAL) : a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196 (12) :1617-26. , URL: https://www.ncbi.nlm.nih.gov/pubmed/12486104?dopt=
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The antibody NAP-03 recognizes a defined intracellular epitope (aa 196-212) of human Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein associating with lipid raft components in the plasma membrane of B cells, NK cells and myeloid cells.
Applications Notes
Immunohistochemistry (paraffin sections) : Recommended dilution: 5 μg/ml; positive tissue: tonsil, germinal center. Western blotting: Recommended dilution: 1-2 μg/ml, incubation 60 min; positive control: RAMOS human lymphoma cell line; non-reducing conditions, 12% separating SDS-PAGE gel.
Isotype
Mouse IgG2a
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