Anti-STAT1 Purified

STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.

Product Specifications

Certification

RUO

Reactivity

Mouse, Human

Immunogen

STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE) .

Target Antigen

STAT1

Clone

SM1

Applications

IP, WB, IHC-P

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kovarik J, Boudny V, Kocak I, Lauerova L, Fait V, Vagundova M: Malignant melanoma associates with deficient IFN-induced STAT 1 phosphorylation. Int J Mol Med. 2003 Sep;12 (3) :335-40. , URL: https://www.ncbi.nlm.nih.gov/pubmed/12883649?dopt=, *Boudny V, Kocak I, Lauerova L, Kovarik J: Interferon inducibility of STAT 1 activation and its prognostic significance in melanoma patients. Folia Biol (Praha) . 2003;49 (4) :142-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/12971583?dopt=

Other References

*Kovarik P, Stoiber D, Eyers PA, Menghini R, Neininger A, Gaestel M, Cohen P, Decker T: Stress-induced phosphorylation of STAT1 at Ser727 requires p38 mitogen-activated protein kinase whereas IFN-gamma uses a different signaling pathway. Proc Natl Acad Sci U S A. 1999 Nov 23;96 (24) :13956-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/10570180?ordin, *Frank DA: STAT signaling in the pathogenesis and treatment of cancer. Mol Med. 1999 Jul;5 (7) :432-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/10449805?ordin, *Bromberg J, Darnell JE Jr: The role of STATs in transcriptional control and their impact on cellular function. Oncogene. 2000 May 15;19 (21) :2468-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/10851045?ordin, *Soond SM, Townsend PA, Barry SP, Knight RA, Latchman DS, Stephanou A: ERK and the E3 ubiquitin ligase beta TRCP targets STAT1 for degradation. J Biol Chem. 2008 Mar 31, URL: https://www.ncbi.nlm.nih.gov/pubmed/18378670?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 5 μg/ml; positive tissue: lung macrophages.Western blotting: Recommended dilution 1-2 μg/ml.