Anti-Hu CD42b Purified

CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.

Product Specifications

Certification

RUO

Reactivity

Non-human primates, Human

Immunogen

Peripheral blood mononuclear cells of a patient suffering with CLL.

Target Antigen

CD42b

Clone

HIP1

Applications

FC (QC tested), IHC-F

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Leukocyte Typing IV., Knapp W et al. (Eds.), Oxford University Press (1989) ., URL: , *Takahashi R, Sekine N, Nakatake T: Influence of monoclonal antiplatelet glycoprotein antibodies on in vitro human megakaryocyte colony formation and proplatelet formation. Blood. 1999 Mar 15;93 (6) :1951-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/10068668?ordin

Other References

*Bergmeier W, Piffath CL, Goerge T, Cifuni SM, Ruggeri ZM, Ware J, Wagner DD: The role of platelet adhesion receptor GPIbalpha far exceeds that of its main ligand, von Willebrand factor, in arterial thrombosis. Proc Natl Acad Sci U S A. 2006 Nov 7;103 (45) :16900-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/17075060?ordin, *Vanhoorelbeke K, Ulrichts H, Van de Walle G, Fontayne A, Deckmyn H: Inhibition of platelet glycoprotein Ib and its antithrombotic potential. Curr Pharm Des. 2007;13 (26) :2684-97., URL: https://www.ncbi.nlm.nih.gov/pubmed/17897012?ordin, *Jain S, Zuka M, Liu J, Russell S, Dent J, Guerrero JA, Forsyth J, Maruszak B, Gartner TK, Felding-Habermann B, Ware J. Platelet glycoprotein Ib alpha supports experimental lung metastasis. Proc Natl Acad Sci U S A. 2007 May 22;104 (21) :9024-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17494758?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody HIP1 reacts with an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa) ; CD42b/c forms a noncovalent complex with CD42a and CD42d.