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Anti-HLA Class I Purified

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Product Specifications

Certification

RUO

Immunogen

COS-7 African green monkey kidney cells

Target Antigen

HLA Class I

Clone

MEM-123

Applications

FC (QC tested), IP, ELISA

Concentration

1 mg/mL

Format

Purified

Buffer

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-123 reacts with an extracellular epitope of all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. The antibody MEM-123 completely blocks binding of classical W6/32 to surface-expressed HLA-G, but does not cross-blocks the antibody MEM-G/9.

Applications Notes

Flow cytometry: Recommended dilution: 4 μg/ml.

Species

Cow, Human, Non-human primates

CAS Number

9007-83-4

Isotype

Mouse IgG3

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