Anti-HLA-E Purified

*Spaans VM, Peters AA, Fleuren GJ, Jordanova ES: HLA-E expression in cervical adenocarcinomas: association with improved long-term survival. J Transl Med. 2012 Sep 4;10:184., URL: https://www.ncbi.nlm.nih.gov/pubmed/22947189 , *Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep;13 (9) :822-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=tremante+e+cerboni+c , *Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64 (3) :315-26. , URL: https://www.ncbi.nlm.nih.gov/pubmed/12590976?dopt=, *Dambaeva SV, Bondarenko GI, Grendell RL, Kravitz RH, Durning M, Golos TG: Non-classical MHC-E (Mamu-E) expression in the rhesus monkey placenta. Placenta. 2008 Jan;29 (1) :58-70., URL: http://www.ncbi.nlm.nih.gov/pubmed/17996936

The antibody MEM-E/02 specifically reacts with an extracellular epitope on denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types.

Immunohistochemistry (paraffin sections) : Recommended dilution: 5-10 μg/ml; positive control: human placenta; heat antigen retrieval (sodium citrate), incubation with mAb: 1 hour / RT, detection DAB.