Anti-Hu CD71 Purified

CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

KG1 human acute myelogenous leukaemia cell line

Target Antigen

CD71

Clone

MEM-189

Applications

WB, FC (QC tested), IP

Concentration

1 mg/mL

Format

Purified

Buffer

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

References & Citations

*Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8 (395) :rs11, URL: http://www.ncbi.nlm.nih.gov/pubmed/26396269

Other References

*Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World J Gastroenterol. 2007 Sep 21;13 (35) :4725-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/17729394?ordin, *Graham RM, Reutens GM, Herbison CE, Delima RD, Chua AC, Olynyk JK, Trinder D: Transferrin receptor 2 mediates uptake of transferrin-bound and non-transferrin-bound iron. J Hepatol. 2008 Feb;48 (2) :327-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/18083267?ordin, *Taketani S: Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation. Tohoku J Exp Med. 2005 Apr;205 (4) :297-318., URL: https://www.ncbi.nlm.nih.gov/pubmed/15750326?ordin, *Rouault TA: How mammals acquire and distribute iron needed for oxygen-based metabolism. PLoS Biol. 2003 Dec;1 (3) :E79., URL: https://www.ncbi.nlm.nih.gov/pubmed/14691550?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-189 reacts with an extracellular epitope on CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor.