Anti-NHERF1 Purified

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin) -binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Bacterially produced recombinant full-length human NHERF1.

Target Antigen

NHERF1

Clone

EBP-10

Applications

IP, WB, IHC-P

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*He P, Zhao L, No YR, Karvar S, Yun CC: The NHERF1 PDZ1 domain and IRBIT interact and mediate the activation of Na+/H+ exchanger 3 by ANG II. Am J Physiol Renal Physiol. 2016 Aug 1;311 (2) :F343-51., URL: https://pubmed.ncbi.nlm.nih.gov/27279487/

Other References

*Lederer ED, Khundmiri SJ, Weinman EJ: Role of NHERF-1 in regulation of the activity of Na-K ATPase and sodium-phosphate co-transport in epithelial cells. J Am Soc Nephrol. 2003 Jul;14 (7) :1711-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/12819230?ordin, *Brdickova N, Brdicka T, Andera L, Spicka J, Angelisova P, Milgram SL, Horejsi V: Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton. FEBS Lett. 2001 Oct 26;507 (2) :133-6., URL: https://pubmed.ncbi.nlm.nih.gov/11684085/, *Weinman EJ, Cunningham R, Wade JB, Shenolikar S: The role of NHERF-1 in the regulation of renal proximal tubule sodium-hydrogen exchanger 3 and sodium-dependent phosphate cotransporter 2a. J Physiol. 2005 Aug 15;567 (Pt 1) :27-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/15932893?ordin, *Wheeler D, Sneddon WB, Wang B, Friedman PA, Romero G: NHERF-1 and the cytoskeleton regulate the traffic and membrane dynamics of G protein-coupled receptors. J Biol Chem. 2007 Aug 24;282 (34) :25076-87., URL: https://www.ncbi.nlm.nih.gov/pubmed/17599914?ordin, *Morales FC, Takahashi Y, Momin S, Adams H, Chen X, Georgescu MM: NHERF1/EBP50 head-to-tail intramolecular interaction masks association with PDZ domain ligands. Mol Cell Biol. 2007 Apr;27 (7) :2527-37., URL: https://www.ncbi.nlm.nih.gov/pubmed/17242191?ordin, *Kreimann EL, Morales FC, de Orbeta-Cruz J, Takahashi Y, Adams H, Liu TJ, McCrea PD, Georgescu MM: Cortical stabilization of beta-catenin contributes to HERF1/EBP50 tumor suppressor function. Oncogene. 2007 Aug 9;26 (36) :5290-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/17325659?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody EBP-10 reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 5 μg/ml. Western blotting: Recommended dilution: 2 μg/ml, reducing conditions, positive control: MCF-7 cell line