Anti-SIT Purified

SIT is a 21 kDa transmembrane adaptor protein expressed exclusively in lymphoid organs. It acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Bacterially produced recombinant intracellular fragment of human SIT.

Target Antigen

SIT

Clone

SIT-01

Applications

IP, WB, FC-IC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107 (1) :213-21., URL: https://pubmed.ncbi.nlm.nih.gov/16160011/

Other References

*Hubener C, Mincheva A, Lichter P, Schraven B, Bruyns E: Complete sequence, genomic organization, and chromosomal localization of the human gene encoding the SHP2-interacting transmembrane adaptor protein (SIT) . Immunogenetics. 2001 May-Jun;53 (4) :337-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/11491537?dopt=, *Simeoni L, Posevitz V, Kolsch U, Meinert I, Bruyns E, Pfeffer K, Reinhold D, Schraven B: The transmembrane adapter protein SIT regulates thymic development and peripheral T-cell functions. Mol Cell Biol. 2005 Sep;25 (17) :7557-68., URL: https://www.ncbi.nlm.nih.gov/pubmed/16107703?dopt=, *Marie-Cardine A, Kirchgessner H, Bruyns E, Shevchenko A, Mann M, Autschbach F, Ratnofsky S, Meuer S, Schraven B: SHP2-interacting transmembrane adaptor protein (SIT), a novel disulfide-linked dimer regulating human T cell activation. J Exp Med. 1999 Apr 19;189 (8) :1181-94., URL: https://www.ncbi.nlm.nih.gov/pubmed/10209036?dopt=

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT.