Anti-TRIM Purified

TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.

Product Specifications

Certification

RUO

Negative Species

Mouse

Reactivity

Human

Immunogen

Recombinant intracellular domain (aa 29-186) of human TRIM.

Target Antigen

TRIM

Clone

TRIM-04

Applications

IP, WB, FC-IC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kirchgessner H, Dietrich J, Scherer J, Isomaki P, Korinek V, Hilgert I, Bruyns E, Leo A, Cope AP, Schraven B.: The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain. J Exp Med. 2001 Jun 4;193 (11) :1269-84. , URL: https://www.ncbi.nlm.nih.gov/pubmed/11390434?dopt=

Other References

*Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107 (1) :213-21., URL: https://pubmed.ncbi.nlm.nih.gov/16160011/, *Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17 (1) :35-49., URL: https://pubmed.ncbi.nlm.nih.gov/15582487/, *Huynh T, Wurch A, Bruyns E, Korinek V, Schraven B, Eichmann K: Developmentally regulated expression of the transmembrane adaptor protein trim in fetal and adult T cells. Scand J Immunol. 2001 Jul-Aug;54 (1-2) :146-54., URL: https://www.ncbi.nlm.nih.gov/pubmed/11439161?dopt=, *Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4 (8) :603-16., URL: https://pubmed.ncbi.nlm.nih.gov/15286727/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.