Anti-p21Waf1 Purified

The tumour suppressor p21Waf1 (wild-type p53-activated fragment 1; also known as Cip1, Cdk interacting protein, or SDI 1) is a cyclin-dependent kinase (Cdk) inhibitor, which is expressed by involvement of p53, Egr-1, AP2, STATs or other transcription factors upon various stimuli resulting in cell cycle arrest. Through its N-terminal domain p21Waf1 inhibits Cdk activity, whereas through the C-terminal domain it inhibits the activity of PCNA (proliferating cell nuclear antigen) to activate DNA replication. Cytosolic location of p21 counteracts its inhibitory activities.

Product Specifications

Certification

RUO

Reactivity

Human, Non-human primates

Immunogen

Human p21 protein

Target Antigen

P21Waf1

Clone

WA-1

Applications

IHC-P, ICC, ELISA

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Kolář Z, Murray PG, Scott K, Harrison A, Vojtěšek B, Dušek J: Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer., URL: https://www.ncbi.nlm.nih.gov/pubmed/10884916?dopt=

Other References

*Choi BH, Kim CG, Bae YS, Lim Y, Lee YH, Shin SY: p21 Waf1/Cip1 expression by curcumin in U-87MG human glioma cells: role of early growth response-1 expression. Cancer Res. 2008 Mar 1;68 (5) :1369-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/18316600?ordin, *Keeshan K, Cotter TG, McKenna SL: Bcr-Abl upregulates cytosolic p21WAF-1/CIP-1 by a phosphoinositide-3-kinase (PI3K) -independent pathway. Br J Haematol. 2003 Oct;123 (1) :34-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/14510940?ordin, *Waga S, Hannon GJ, Beach D, Stillman B: The p21 inhibitor of cyclin-dependent kinases controls DNA replication by interaction with PCNA. Nature. 1994 Jun 16;369 (6481) :574-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/7911228?ordina, *Horky M, Kuchtickova S, Vojtesek B, Kolar F: Induction of cell-cycle inhibitor p21 in rat ventricular myocytes during early postnatal transition from hyperplasia to hypertrophy. Physiol Res. 1997;46 (3) :233-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/9728512?dopt=A, *Zhang C, Kavurma MM, Lai A, Khachigian LM: Ets-1 protects vascular smooth muscle cells from undergoing apoptosis by activating p21WAF1/Cip1: ETS-1 regulates basal and and inducible p21WAF1/Cip: ETS-1 regulates basal and inducible p21WAF1/Cip1 transcription via distinct cis-acting elements in the p21WAF/Cip1 promoter. J Biol Chem. 2003 Jul 25;278 (30) :27903-9. , URL: https://www.ncbi.nlm.nih.gov/pubmed/12740370?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1; intracellular antigen), a 21 kDa tumour suppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferating cell nuclear antigen.