Anti-Hu CD41 Purified

CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Leukocytes of patient suffering from LGL-type leukaemia.

Target Antigen

CD41

Clone

MEM-06

Applications

FC (QC tested)

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Belizaire RM, Prakash PS, Richter JR, Robinson BR, Edwards MJ, Caldwell CC, Lentsch AB, Pritts TA: Microparticles from stored red blood cells activate neutrophils and cause lung injury after hemorrhage and resuscitation. J Am Coll Surg. 2012 Apr;214 (4) :648-55, URL: https://pubmed.ncbi.nlm.nih.gov/22342784/, *Pagani G, Pereira JPV, Stoldt VR, Beck A, Scharf RE, Gohlke H: The human platelet antigen-1b (Pro33) variant of α IIbβ3 allosterically shifts the dynamic conformational equilibrium of this integrin toward the active state. J Biol Chem. 2018 Mar 30;293 (13) :4830-4844., URL: https://pubmed.ncbi.nlm.nih.gov/29462793/

Other References

*Mitjavila-Garcia MT, Cailleret M, Godin I, Nogueira MM, Cohen-Solal K, Schiavon V, Lecluse Y, Le Pesteur F, Lagrue AH, Vainchenker W: Expression of CD41 on hematopoietic progenitors derived from embryonic hematopoietic cells. Development. 2002 Apr;129 (8) :2003-13., URL: https://www.ncbi.nlm.nih.gov/pubmed/11934866?ordin, *Ferkowicz MJ, Starr M, Xie X, Li W, Johnson SA, Shelley WC, Morrison PR, Yoder MC: CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo. Development. 2003 Sep;130 (18) :4393-403., URL: https://www.ncbi.nlm.nih.gov/pubmed/12900455?ordin, *Zhang J, Varas F, Stadtfeld M, Heck S, Faust N, Graf T: CD41-YFP mice allow in vivo labeling of megakaryocytic cells and reveal a subset of platelets hyperreactive to thrombin stimulation. Exp Hematol. 2007 Mar;35 (3) :490-499., URL: https://www.ncbi.nlm.nih.gov/pubmed/17309829?ordin, *Debili N, Robin C, Schiavon V, Letestu R, Pflumio F, Mitjavila-Garcia MT, Coulombel L, Vainchenker W: Different expression of CD41 on human lymphoid and myeloid progenitors from adults and neonates. Blood. 2001 Apr 1;97 (7) :2023-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/11264167?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-06 reacts with an extracellular epitope of CD41 (GPIIb), a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain; 120 kDa) and GPIIb beta (light chain; 23 kDa) . CD41 is mainly expressed on platelets and megakaryocytes.