Anti-Hu CD21 Purified

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Product Specifications

Certification

RUO

Reactivity

Pig, Human, Dog, Cow

Immunogen

IM9 human B-lymphoblastoid cell line

Target Antigen

CD21

Clone

LT21

Applications

FC (QC tested), IHC-F

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997) ., URL: , *Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs. Vet Immunol Immunopathol. 2007 Sep 15;119 (1-2) :56-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/17673300?ordin, *Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319 (1-2) :21-33., URL: http://www.ncbi.nlm.nih.gov/pubmed/17098248

Other References

*Singh A, Blank M, Shoenfeld Y, Illges H: Antiphospholipid syndrome patients display reduced titers of soluble CD21 in their sera irrespective of circulating anti-beta2-glycoprotein-I autoantibodies. Rheumatol Int. 2008 Jan 3, URL: https://www.ncbi.nlm.nih.gov/pubmed/18172655?ordin, *Zabel MD, Heikenwalder M, Prinz M, Arrighi I, Schwarz P, Kranich J, von Teichman A, Haas KM, Zeller N, Tedder TF, Weis JH, Aguzzi A: Stromal complement receptor CD21/35 facilitates lymphoid prion colonization and pathogenesis. J Immunol. 2007 Nov 1;179 (9) :6144-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/17947689?ordin, *Kasprzak A, Spachacz R, Wachowiak J, Stefanska K, Zabel M: Epstein-Barr virus (EBV) infection in B-cell non-Hodgkin's lymphomas in children: virus latency and its correlation with CD21 and CD23 molecules. Folia Histochem Cytobiol. 2007;45 (3) :169-79., URL: https://www.ncbi.nlm.nih.gov/pubmed/17951165?ordin, *Twohig J, Kulik L, Haluszczak C, Reuter J, Rossbach A, Bull M, Holers VM, Marchbank KJ: Defective B cell ontogeny and immune response in human complement receptor 2 (CR2, CD21) transgenic mice is partially recovered in the absence of C3. Mol Immunol. 2007 Jul;44 (13) :3434-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/17379312?ordin, *Roozendaal R, Carroll MC: Complement receptors CD21 and CD35 in humoral immunity. Immunol Rev. 2007 Oct;219:157-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/17850488?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody LT21 reacts with an extracellular epitope of CD21 (CR2), a 145 kDa transmembrane glycoprotein (complement C3d receptor - C3dR) expressed on B lymphocytes, follicular dendritic cells, some epithelial cells and a subsets of T lymphocytes. It is not expressed on immature B cells.