Anti-VCP Purified

VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Product Specifications

Certification

RUO

Reactivity

Mouse, Human

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Target Antigen

VCP

Clone

Hs-14

Applications

FC (QC tested), WB, ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

References & Citations

*Tepla O. et al. In: In vitro Fertilization & Human Reproductive Genetics, ed. Monduzzi, Bologna, 107, (1999) ., URL: , *Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J: Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization. Ceska Gynekol. 2000 Jan;65 (1) :28-32. Czech., URL: https://www.ncbi.nlm.nih.gov/pubmed/10750294?dopt=, *Peknicova J, Chladek D, Hozak P: Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol. 2005 Jan;53 (1) :42-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/15667524?dopt=

Other References

*Pavlásek J, Peknicová J, Ulcová-Gallová Z, Nováková P, Reischig J, Micanová Z, Rokyta Z: Significance of determination of intra-acrosomal proteins and sperm antibodies in human reproduction. Ceska Gynekol. 2004 Jul;69 (4) :306-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/15369252?ordin, *Peknicova J, Kyselova V, Buckiova D, Boubelik M: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47 (5) :311-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/12148547?dopt=

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.

Applications Notes

Immunocytochemistry: Recommended dilution: 10 μg/ml, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought) . Flow cytometry: Intraacrosomal staining; recommended dilution: 3-12 µg/ml.