Anti-Can Intra-Acrosomal Protein Purified
One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Product Specifications
Certification
RUO
Reactivity
Dog
Immunogen
Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
Target Antigen
Intra-Acrosomal Protein
Clone
Ds-2
Applications
ICC, WB
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Geussova G, Peknicova J, Capkova J, Kalab P, Moos J, Philimonenko VV, Hozak P: Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction. Andrologia. 1997 Sep-Oct;29 (5) :261-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/9350326?dopt=A
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The mouse monoclonal antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.
Applications Notes
Immunocytochemistry: Membrane permeabilization (acetone) is essential.
Isotype
Mouse IgG
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