Anti-Hu CD98 Purified

CD98 (4F2) is a type II transmembrane glycoprotein which serves as the heavy chain of the heterodimeric amino acid transporters (HATs) . CD98, linked to various light chains by disulfide bond, is responsible for cell surface expression and basolateral localization of this transporter complex in polarized epithelial cells and also interacts with beta1 integrins and increases their affinity for ligand. Besides its roles in amino acid transport, CD98 is thus involved in cell fusion and activation. It is implicated in regulation of cellular differentiation, growth and apoptosis.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

RAJI human Burkitt's lymphoma cell line

Target Antigen

CD98

Clone

MEM-108

Applications

FC (QC tested), IP, IHC-P

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20 (8) :1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/

Other References

*Dalton P, Christian HC, Redman CW, Sargent IL, Boyd CA: Differential effect of cross-linking the CD98 heavy chain on fusion and amino acid transport in the human placental trophoblast (BeWo) cell line. Biochim Biophys Acta. 2007 Mar;1768 (3) :401-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/17258169?ordin, *Cai S, Bulus N, Fonseca-Siesser PM, Chen D, Hanks SK, Pozzi A, Zent R: CD98 modulates integrin beta1 function in polarized epithelial cells. J Cell Sci. 2005 Mar 1;118 (Pt 5) :889-99., URL: https://www.ncbi.nlm.nih.gov/pubmed/15713750?ordin, *Cho JY, Skubitz KM, Katz DR, Chain BM: CD98-dependent homotypic aggregation is associated with translocation of protein kinase Cdelta and activation of mitogen-activated protein kinases. Exp Cell Res. 2003 May 15;286 (1) :1-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/12729789?ordin, *Liu X, Charrier L, Gewirtz A, Sitaraman S, Merlin D: CD98 and intracellular adhesion molecule I regulate the activity of amino acid transporter LAT-2 in polarized intestinal epithelia. J Biol Chem. 2003 Jun 27;278 (26) :23672-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/12716892?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-108 reacts with an extracellular epitope of CD98, a 125 kDa disulfide-linked heterodimer (80 kDa glycosylated heavy chain + 45 kDa non-glykosylated light chain) . CD98 is expressed on T lymphocytes (upon activation) and activated NK cells; it is also present at low levels on B lymphocytes, NK cells, monocytes and platelets.