Anti-alpha-Fetoprotein Purified
Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.
Product Specifications
Certification
RUO
Immunogen
Purified alpha-Fetoprotein
Target Antigen
Alpha-Fetoprotein
Clone
AFP-01
Applications
IP, ELISA, WB, ICC
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Stefanova I, Horejsi V, Kristofova H, Angelisova P, Zizkovsky V, Hilgert I: Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J Immunol Methods. 1988 Jun 28;111 (1) :67-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/2455753?dopt=A, *Cavin LG, Venkatraman M, Factor VM, Kaur S, Schroeder I, Mercurio F, Beg AA, Thorgeirsson SS, Arsura M: Regulation of alpha-fetoprotein by nuclear factor-kappaB protects hepatocytes from tumor necrosis factor-alpha cytotoxicity during fetal liver development and hepatic oncogenesis. Cancer Res. 2004 Oct 1;64 (19) :7030-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15466196?dopt=, *Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277 (10) :7955-61., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=%C4%8Derm%C3%A1k%20l%20%C5%A1%C3%ADmov%C3%A1%20%C5%A1%20pintzas%20a , *Green CJ, Charlton CA, Wang LM, Silva M, Morten KJ, Hodson L: The isolation of primary hepatocytes from human tissue: optimising the use of small non-encapsulated liver resection surplus. Cell Tissue Bank 2017 Dec;18 (4) :597-604., URL: https://pubmed.ncbi.nlm.nih.gov/28717878/, *Colquhoun DR, Goldman LR, Cole RN, Gucek M, Mansharamani M, Witter FR, Apelberg BJ, Halden RU: Global screening of human cord blood proteomes for biomarkers of toxic exposure and effect. Environ Health Perspect. 2009 May;117 (5) :832-8., URL: https://pubmed.ncbi.nlm.nih.gov/19478969/, *Wang Z, Bi B, Song H, Liu L, Zheng H, Wang S, Shen Z: Proliferation of human hepatocellular carcinoma cells from surgically resected specimens under conditionally reprogrammed culture. Mol Med Rep. 2019 Jun;19 (6) :4623-4630., URL: https://pubmed.ncbi.nlm.nih.gov/31059040/
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The mouse monoclonal antibody AFP-01 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
Species
Human
CAS Number
9007-83-4
Isotype
Mouse IgG1
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items