Anti-Hu Ig Kappa Light Chain Purified
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa) . Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Product Specifications
Certification
RUO
Reactivity
Human
Immunogen
Crude thymus membrane fraction.
Target Antigen
Ig Kappa Light Chain
Clone
MEM-09
Applications
FC (QC tested), IP, WB, IHC-P, ICC, ELISA
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Horejsi V, Hilgert I, Kristofova H, Bazil V, Bukovsky A, Kulhankova J: Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) . 1986;32 (1) :12-25. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2422063?dopt=A
Other References
*Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh) . 1975;194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690?ordinalp
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The antibody MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa) . Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM.
Applications Notes
Flow cytometry: Recommended dilution: 1-5 μg/ml.
Isotype
Mouse IgG1
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