Anti-HLA-DQ1/DQ3 Purified
The antibody HL-37 reacts with polymorphic determinant on human HLA-DQ1 and HLA-DQ3 molecules (recognized epitope was found on isolated beta chain of DQ1), but does not react with HLA-DQ2. DQ is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages) .
Product Specifications
Certification
RUO
Reactivity
Pig, Human
Immunogen
Burkitt's lymphoma cell line Raji.
Target Antigen
HLA-DQ1/DQ3
Clone
HL-37
Applications
FC (QC tested), IP, WB
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Horejsi V, Nemec M, Angelisova P, Kristofova H, Gorga JC, Hilgert I: Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens. 1986 Nov;28 (5) :288-97. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2435017?dopt=A
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The antibody HL-37 reacts with extracellular polymorphic determinant on human HLA-DQ1 and HLA-DQ3 molecules (recognized epitope was found on isolated beta chain of DQ1), but does not react with HLA-DQ2. DQ is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages) .
Applications Notes
Flow cytometry: Recommended dilution: 1-4 μg/ml. Western blotting: non-reducing conditions.
Isotype
Mouse IgG3
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