Anti-Hu CD99R Purified

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

HPB-ALL human peripheral blood leukemia T-cell line

Target Antigen

CD99R

Clone

MEM-131

Applications

FC (QC tested), IP, WB, IHC-P

Concentration

1 mg/mL

Format

Purified

Buffer

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

References & Citations

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995) ., URL: , *Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995 May 1;85 (9) :2402-13., URL: https://www.ncbi.nlm.nih.gov/pubmed/7537112, *Cermak L, Simova S, Pintzas A, Horejsi V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277 (10) :7955-61. , URL: https://www.ncbi.nlm.nih.gov/pubmed/11773067?dopt=

Other References

*Bernard G, Raimondi V, Alberti I, Pourtein M, Widjenes J, Ticchioni M, Bernard A: CD99 (E2) up-regulates alpha4beta1-dependent T cell adhesion to inflamed vascular endothelium under flow conditions. Eur J Immunol. 2000 Oct;30 (10) :3061-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/11069091?ordin, *Bernard G, Breittmayer JP, de Matteis M, Trampont P, Hofman P, Senik A, Bernard A: Apoptosis of immature thymocytes mediated by E2/CD99. J Immunol. 1997 Mar 15;158 (6) :2543-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/9058785?ordina, *Bernard G, Zoccola D, Deckert M, Breittmayer JP, Aussel C, Bernard A: The E2 molecule (CD99) specifically triggers homotypic aggregation of CD4+ CD8+ thymocytes. J Immunol. 1995 Jan 1;154 (1) :26-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/7527813?ordina, *Gelin C, Aubrit F, Phalipon A, Raynal B, Cole S, Kaczorek M, Bernard A: The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product. EMBO J. 1989 Nov;8 (11) :3253-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/2479542?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-131 reacts with CD99R, an extracellular epitope restricted to a subset of CD99 molecule expressed on myeloid cells, NK cells and T lymphocytes.

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: thymic lymphocytes. Flow cytometry: Recommended dilution: 1 μg/ml. Western blotting: Non-reducing conditions.