Anti-Hu CD59 Purified

CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC) . It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.

Product Specifications

Certification

RUO

Reactivity

Mouse, Human

Immunogen

Thymocytes and T lymphocytes

Target Antigen

CD59

Clone

MEM-43/5

Applications

WB, FC (QC tested), IP, IHC-P

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1;272 (31) :19242-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9235917, *Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19 (5) :675-84., URL: https://www.ncbi.nlm.nih.gov/pubmed/17446208?itool, *Bodian DL, Davis SJ, Morgan BP, Rushmere NK: Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59. J Exp Med. 1997 Feb 3;185 (3) :507-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/9053451?dopt=A, *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995) ., URL:

Other References

*Baalasubramanian S, Harris CL, Donev RM, Mizuno M, Omidvar N, Song WC, Morgan BP: CD59a is the primary regulator of membrane attack complex assembly in the mouse. J Immunol. 2004 Sep 15;173 (6) :3684-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/15356114?ordin, *Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE: CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation. J Immunol. 1994 Sep 15;153 (6) :2444-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/7521361?ordina, *Rooney IA, Davies A, Griffiths D, Williams JD, Davies M, Meri S, Lachmann PJ, Morgan BP: The complement-inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells. Clin Exp Immunol. 1991 Feb;83 (2) :251-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/1704296?ordina, *Meri S, Morgan BP, Davies A, Daniels RH, Olavesen MG, Waldmann H, Lachmann PJ: Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers. Immunology. 1990 Sep;71 (1) :1-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/1698710?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI) -anchored extracellular glycoprotein expressed on the surface of all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies.

Applications Notes

Flow cytometry: Recommended dilution: 0.5-4 μg/ml; positive control: blood. Immunohistochemistry (paraffin sections) : Recommended dilution: 5 μg/ml. Western blotting: Recommended dilution: 1-2 μg/ml; positive control: mouse spleen, non-reducing conditions.