Anti-Hu CD55 Purified

CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.

Product Specifications

Certification

RUO

Reactivity

Human, Non-human primates

Immunogen

HPB-ALL human T cell line

Target Antigen

CD55

Clone

MEM-118

Applications

FC (QC tested), IP, IHC-P, WB

Concentration

1 mg/mL

Format

Purified

Buffer

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

References & Citations

*Angelisová P, Drbal K, Horejsí V, Cerný J: Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase. Blood. 1999 Feb 15;93 (4) :1437-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/10075459?itool=

Other References

*Miwa T, Maldonado MA, Zhou L, Sun X, Luo HY, Cai D, Werth VP, Madaio MP, Eisenberg RA, Song WC: Deletion of decay-accelerating factor (CD55) exacerbates autoimmune disease development in MRL/lpr mice. Am J Pathol. 2002 Sep;161 (3) :1077-86., URL: https://www.ncbi.nlm.nih.gov/pubmed/12213736?ordin, *Mikesch JH, Buerger H, Simon R, Brandt B: Decay-accelerating factor (CD55) : a versatile acting molecule in human malignancies. Biochim Biophys Acta. 2006 Aug;1766 (1) :42-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/16784816?ordin, *Abbott RJ, Spendlove I, Roversi P, Fitzgibbon H, Knott V, Teriete P, McDonnell JM, Handford PA, Lea SM: Structural and functional characterization of a novel T cell receptor co-regulatory protein complex, CD97-CD55. J Biol Chem. 2007 Jul 27;282 (30) :22023-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/17449467?ordin, *Miwa T, Maldonado MA, Zhou L, Yamada K, Gilkeson GS, Eisenberg RA, Song WC: Decay-accelerating factor ameliorates systemic autoimmune disease in MRL/lpr mice via both complement-dependent and -independent mechanisms. Am J Pathol. 2007 Apr;170 (4) :1258-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/17392165?ordin, *VanLandingham JW, Cekic M, Cutler S, Hoffman SW, Stein DG: Neurosteroids reduce inflammation after TBI through CD55 induction. Neurosci Lett. 2007 Sep 25;425 (2) :94-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17826908?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI) -anchored single chain extracellular glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells; it is weakly present on NK cells.

Applications Notes

Flow cytometry: Recommended dilution: 10 μg/ml. Immunohistochemistry (paraffin sections) : Heat-mediated antigen retrieval, recommended dilution: 10 μg/ml.