Anti-Hu CD54 Purified

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1) . The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Product Specifications

Certification

RUO

Reactivity

Human, Cow, Rat

Immunogen

Raji human Burkitt's lymphoma cell line

Target Antigen

CD54

Clone

MEM-111

Applications

WB, IHC-P, ELISA, FC (QC tested), ICC

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Nagaraju K et al.: A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli. Clin Exp Immunol 113, 407 (1998) ., URL: https://www.ncbi.nlm.nih.gov/pubmed/9737670?dopt=A, *Tachimoto H. et al., Eotaxin-2 alters eosinophil integrin function via mitogen-activated protein kinases. Am J Respir Cell Mol Biol 26, 645 (2002) ., URL: https://pubmed.ncbi.nlm.nih.gov/12034562/, *Burdick MM et al.: Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow. Am J Physiol Cell Physiol 284, C977 (2003) ., URL: https://www.physiology.org/doi/full/10.1152/ajpcel, *Hertel L et al.: Susceptibility of Immature and Mature Langerhans Cell-Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus. J Virol. 77, 7563 (2003) ., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC16478, *Bacáková L, Mares V, Lysá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun;21 (11) :1173-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10817270?ordin, *Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15;179 (12) :8128-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/18056355, *Bacáková L, Mares V, Lysá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun;21 (11) :1173-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10602070?ordin, *Leeuwenberg JFM et al.: E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77, 543 (1992) ., URL: https://www.ncbi.nlm.nih.gov/pubmed/1283598?dopt=A, *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997) ., URL: , *Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15;179 (12) :8128-36., URL: https://pubmed.ncbi.nlm.nih.gov/18056355/

Other References

*Boyd AW, Wawryk SO, Burns GF, Fecondo JV: Intercellular adhesion molecule 1 (ICAM-1) has a central role in cell-cell contact-mediated immune mechanisms. Proc Natl Acad Sci U S A. 1988 May;85 (9) :3095-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/3362863?dopt=A, *Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO: Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation. Blood. 1989 May 15;73 (7) :1896-903., URL: https://www.ncbi.nlm.nih.gov/pubmed/2469503?dopt=A, *Springer TA: Adhesion receptors of the immune system. Nature. 1990 Aug 2;346 (6283) :425-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/1974032?dopt=A, *Ockenhouse CF, Betageri R, Springer TA, Staunton DE: Plasmodium falciparum-infected erythrocytes bind ICAM-1 at a site distinct from LFA-1, Mac-1, and human rhinovirus. Cell. 1992 Jan 10;68 (1) :63-9. Erratum in: Cell 1992 Mar 6;68 (5) :following 994., URL: https://www.ncbi.nlm.nih.gov/pubmed/1346257?dopt=A

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-111 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) . The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

Applications Notes

Flow cytometry: Recommended dilution: 0.5-4 μg/ml. Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: thymus, RE cells. Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.