Anti-Hu CD53 Purified

CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Leukocytes of pacient suffering from a LGL-type leukemia.

Target Antigen

CD53

Clone

MEM-53

Applications

FC (QC tested), IP, WB, IHC-F

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8 (395) :rs11, URL: http://www.ncbi.nlm.nih.gov/pubmed/26396269 , *Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995 May 1;85 (9) :2402-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/7537112 , *Szollosi J, Horejsi V, Bene L, Angelisova P, Damjanovich S: Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J Immunol. 1996 Oct 1;157 (7) :2939-46. , URL: https://www.ncbi.nlm.nih.gov/pubmed/8816400?dopt=A, *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995) ., URL: , *Rasmussen AM, Blomhoff HK, Stokke T, Horejsi V, Smeland EB: Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol. 1994 Dec 1;153 (11) :4997-5007., URL: https://www.ncbi.nlm.nih.gov/pubmed/7963560?dopt=A, *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989) ., URL: , *Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) . 1989;35 (5) :289-97., URL: https://www.ncbi.nlm.nih.gov/pubmed/2534480?dopt=A

Other References

*Yunta M, Rodríguez-Barbero A, Arévalo MA, López-Novoa JM, Lazo PA: Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primary cultures of mesangial cells. Kidney Int. 2003 Feb;63 (2) :534-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/12631118?ordin, *Yunta M, Lazo PA: Apoptosis protection and survival signal by the CD53 tetraspanin antigen. Oncogene. 2003 Feb 27;22 (8) :1219-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/12606948?ordin, *Kim TR, Yoon JH, Kim YC, Yook YH, Kim IG, Kim YS, Lee H, Paik SG: LPS-induced CD53 expression: a protection mechanism against oxidative and radiation stress. Mol Cells. 2004 Feb 29;17 (1) :125-31., URL: https://www.ncbi.nlm.nih.gov/pubmed/15055538?ordin, *Puls KL, Hogquist KA, Reilly N, Wright MD: CD53, a thymocyte selection marker whose induction requires a lower affinity TCR-MHC interaction than CD69, but is up-regulated with slower kinetics. Int Immunol. 2002 Mar;14 (3) :249-58., URL: https://www.ncbi.nlm.nih.gov/pubmed/11867561?ordin, *Mollinedo F, Martín-Martín B, Gajate C, Lazo PA: Physiological activation of human neutrophils down-regulates CD53 cell surface antigen. J Leukoc Biol. 1998 Jun;63 (6) :699-706., URL: https://www.ncbi.nlm.nih.gov/pubmed/9620662?ordina, *Angelisova P, Vlcek C, Stefanova I, Lipoldova M, Horejsi V: The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens. Immunogenetics. 1990;32 (4) :281-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/1700763?dopt=A, *Olweus J, Lund-Johansen F, Horejsi V: CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol. 1993 Jul 15;151 (2) :707-16. , URL: https://www.ncbi.nlm.nih.gov/pubmed/8335905?dopt=A

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-53 reacts with an extracellular epitope of CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F) .

Applications Notes

Immunohistochemistry (frozen sections) : It is suitable for discrimination of lymphomas from other tumors. Flow cytometry: Recommended dilution: 1-5 μg/ml.