Anti-Hu CD47 Purified

CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

Product Specifications

Certification

RUO

Reactivity

Non-human primates, Human, Pig

Immunogen

COS-7 (African green monkey) cells

Target Antigen

CD47

Clone

MEM-122

Applications

FC (QC tested), WB, IHC-F

Concentration

1 mg/mL

Format

Purified

Buffer

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

References & Citations

*Smith RE, Patel V, Seatter SD, Deehan MR, Brown MH, Brooke GP, Goodridge HS, Howard CJ, Rigley KP, Harnett W, Harnett MM: A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes. Blood. 2003 Oct 1;102 (7) :2532-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/12805067

Other References

*Barazi HO, Li Z, Cashel JA, Krutzsch HC, Annis DS, Mosher DF, Roberts DD: Regulation of integrin function by CD47 ligands. Differential effects on alpha vbeta 3 and alpha 4beta1 integrin-mediated adhesion. J Biol Chem. 2002 Nov 8;277 (45) :42859-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/12218055?ordin, *Murata T, Ohnishi H, Okazawa H, Murata Y, Kusakari S, Hayashi Y, Miyashita M, Itoh H, Oldenborg PA, Furuya N, Matozaki T: CD47 promotes neuronal development through Src- and FRG/Vav2-mediated activation of Rac and Cdc42. J Neurosci. 2006 Nov 29;26 (48) :12397-407., URL: https://www.ncbi.nlm.nih.gov/pubmed/17135401?ordin, *Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47-SIRPalpha signaling in xenograft rejection by macrophages. Proc Natl Acad Sci U S A. 2007 Mar 20;104 (12) :5062-6. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17360380?ordin, *Isenberg JS, Pappan LK, Romeo MJ, Abu-Asab M, Tsokos M, Wink DA, Frazier WA, Roberts DD: Blockade of thrombospondin-1-CD47 interactions prevents necrosis of full thickness skin grafts. Ann Surg. 2008 Jan;247 (1) :180-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/18156939?ordin, *Orazizadeh M, Lee HS, Groenendijk BC, Millward-Sadler J, Wright MO, Lindberg FP, Salter DM. CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model. Arthritis Res Ther. 2008 Jan 10;10 (1) :R4, URL: https://www.ncbi.nlm.nih.gov/pubmed/18186923?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-122 reacts with an extracellular epitope of CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.