Anti-Hu CD6 Purified

Human CD6 antigen purified by immunoaffinity chromatography from HBP-ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone) .

*Castro MA, Oliveira MI, Nunes RJ, Fabre S, Barbosa R, Peixoto A, Brown MH, Parnes JR, Bismuth G, Moreira A, Rocha B, Carmo AM: Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse. J Immunol. 2007 Apr 1;178 (7) :4351-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/17371992?itool=, *Hassan NJ, Simmonds SJ, Clarkson NG, Hanrahan S, Puklavec MJ, Bomb M, Barclay AN, Brown MH: CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76. Mol Cell Biol. 2006 Sep;26 (17) :6727-38., URL: http://www.ncbi.nlm.nih.gov/pubmed/16914752?itool=

*Osorio LM, De Santiago A, Aguilar-Santelises M, Mellstedt H, Jondal M: CD6 ligation modulates the Bcl-2/Bax ratio and protects chronic lymphocytic leukemia B cells from apoptosis induced by anti-IgM. Blood. 1997 Apr 15;89 (8) :2833-41., URL: https://pubmed.ncbi.nlm.nih.gov/9108402/, *Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN, Figdor CG: Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells. Blood. 2006 Apr 15;107 (8) :3212-20. Epub 2005 Dec 13., URL: https://pubmed.ncbi.nlm.nih.gov/16352806/

The antibody MEM-98 reacts with an extracellular epitope of CD6, a 100-130 kDa single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells.

Flow cytometry: Recommended dilution: 2 μg/ml. Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: spleen. Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.