Anti-Hu CD2 Purified

CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Human peripheral T cells.

Target Antigen

CD2

Clone

MEM-65

Applications

FC (QC tested), IP

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Drbal K, Hilgert I, Cebecauer M, Angelisova P, Horejsi V: New monoclonal antibodies to human leucocyte surface molecule CD2. Folia Biol (Praha) . 1997;43 (6) :245-6. , URL: http://www.ncbi.nlm.nih.gov/pubmed/9595269 , *Espagnolle N, Depoil D, Zaru R, Demeur C, Champagne E, Guiraud M, Valitutti S: CD2 and TCR synergize for the activation of phospholipase Cgamma1/calcium pathway at the immunological synapse. Int Immunol. 2007 Mar;19 (3) :239-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/17220479?itool, *Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91 (10) :3901-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9573028?itool=, *Drbal K, Hilgert I, Cebecauer M, Angelisova P, Horejsi V: New monoclonal antibodies to human leucocyte surface molecule CD2. Folia Biol (Praha) . 1997;43 (6) :245-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/9595269, *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997) ., URL:

Other References

*Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13 (31) :4207-13., URL: https://pubmed.ncbi.nlm.nih.gov/17696249/, *Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE: Mechanisms of cellular avidity regulation in CD2-CD58-mediated T cell adhesion. ACS Chem Biol. 2006 Nov 21;1 (10) :649-58., URL: https://pubmed.ncbi.nlm.nih.gov/17168569/, *Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass-supported planar bilayers reconstituted with the immunoglobulin-related adhesion molecule CD58. J Biol Chem. 1997 Jun 20;272 (25) :15782-8., URL: https://pubmed.ncbi.nlm.nih.gov/9188475/, *Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13 (31) :4207-13., URL: https://pubmed.ncbi.nlm.nih.gov/17696249/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-65 recognizes a unique extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T-lymphocytes and NK cells; also expressed by all thymocytes.